The lightโdark box test is frequently used in rodents to assess anxiety-like behavior. The apparatus consists of a dark and a bright lit compartment. It relies on the aversion for brightly
Anxiety-like behaviors in rodents are widely explored in neuroethological apparatuses, such as the light-dark box (LDB) test through different protocols, which have been shown to influence the behavioral outcomes and probably the activation of the hypothalamic-pituitary-adrenal (HPA) axis.
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